Production of Large-volume Cellulase Crystals for Visualization of Hydrogen Atoms

DOI:10.15011//jasma.34.340108
Int. J. Microgravity Sci. Appl. 2017p340108
Author
Mikako TACHIOKA, Akihiko NAKAMURA, Takuya ISHIDA, Sachiko TAKAHASHI, Bin YAN, Hiroaki TANAKA, Naoki FURUBAYASHI, Koji INAKA,Kiyohiko IGARASHI,and Masahiro SAMEJIMA
Organization
Graduate School of Agricultural and Life Sciences, The University of Tokyo,Institute for Molecular Science, National Institute of Natural Science,Confocal Science Inc.,Maruwa Foods and Biosciences Inc., VTT Technical Research Centre of Finland
Abstract
Water molecules play important roles in enzymatic reaction especially for hydrolases, which use water as a substrate. Neutron crystallography is a powerful tool for visualizing hydrogen atoms to understand protonation states of protein and orientation of water molecules, while it requires a large-volume crystal ( >1 mm3 ) compared with X-ray crystallography. To overcome the hurdle of crystal volume, a counter diffusion device equipped with 2 mm diameter can be used at the microgravity experiments. We report here the crystallization of two cellulases which hydrolyse glycosidic linkages in cellulose.
Keyword(s)
Large-volume crystal growth, Microgravity, Neutron crystallography, Cellulase
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Received 15 November 2016, Accepted 14 December 2016, Published 31 January 2017

© The Japan Society of Microgravity Applicaiton

この投稿文は次の言語で読めます: Japanese

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