Fibronectin is Involved in Gravity-Sensing of Osteoblast Like Cell

Int. J. Microgravity Sci. Appl. 2011_s036
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ECM-integrin-CSK system is normally considered as gravity-sensor of cell and most researches focus on the cytoskeleton (CSK). How about the extracellular matrix (ECM) protein? In previous work, we found that fibronectin (FN) gene expression in MG-63 cells under simulated weightlessness condition was increased significantly compared to other conditions and this result was verified by microarray test. Here the FN’s alterations of another human osteoblast like cell hFOB1.19 on the protein level under diverse gravitational condition were investigated. Both cellular FN and soluble FN were detected. More FN was expressed in hFOB1.19 cells medium after co-cultured with RGD-peptide and integrin antibody. These results hint that FN changes in altered gravity maybe involved with the interference of the interaction between FN and integrin.
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